ABSTRACT
Aim: The present study was carried out to investigate the impact of Roundup 41% (glyphosate) on serum enzymes, like aminotransferases activity (ALT and AST), alkaline phosphates (ALP), total protein and glucose of fresh water fish, Rasbora daniconius.Methodology: LC50 of glyphosate for R. daniconius was calculated by static bioassays and recorded as 5.6 ppm at 96 hr. The fish were exposed to sub-lethal concentration (1/10th 96 hr LC50) of glyphosate for a period of 7, 14, 21 and 28 days. The serum ALT, AST, ALP, glucose and total protein were determined by standard methods. Results: Statistically important differences were observed in alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total protein and glucose levles. ALT, AST and ALP values were significantly higher (p<0.05) in treated group, compared with the control group. There was also a significant increase (p<0.05) in the level of serum glucose while the reduced amount of protein content as compared to control fish. Interpretation: Increased metabolic enzymes, protein metabolism and hyperglycemia in the serum may be a possible indicator of liver damage caused by Roundup 41% (glyphosate) exposure in fish, Rasbora daniconius.